3 10 24 https://repository.horizon.ac.id/files/original/0165aeb47d13f314b9753cbe2ffebb61.pdf e6d7493a58da0a9524896fa423b7c912 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Medical Journal of Indonesia Vol. 29 No. 2 2020 FKUI Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020 Optimization of The Apolipoprotein B mRNA Editing Enzyme Catalytic Polypeptide-like-3G (APOBEC3G) Gene to Enhance Its Expression in Escherichia coli Subject The topic of the resource APOBEC3G, codon usage, prokaryote gene expression Description An account of the resource BACKGROUND Apolipoprotein B mRNA editing enzyme catalytic polypeptide-like-3G (APOBEC3G) can abolish HIV infection by inducing lethal mutations in the HIV genome. The HIV protein virion infectivity factor (Vif) can interact with APOBEC3G protein and cause its degradation. Development of a method that can screen substances inhibiting the APOBEC3G-Vif interaction is necessary for identification of substances that potentially used in anti-HIV drug development. In order to increase expression of recombinant APOBEC3G protein that will be used in APOBEC3G-Vif interaction assay, we developed an optimized APOBEC3G gene for expression in Escherichia coli. METHODS The gene coding APOBEC3G was codon-optimized in accordance with prokaryotic codon using DNA 2.0 software to avoid bias codons that could inhibit its expression. The APOBEC3G gene was synthesized and sub-cloned into pQE80L plasmid vector. pQE80L containing APOBEC3G was screened by polymerase chain reaction, enzyme restriction, and sequencing to verify its DNA sequence. The recombinant APOBEC3G was expressed in E. coli under isopropyl-β-D thiogalactoside (IPTG) induction and purified by using nickel-nitrilotriacetic acid (Ni-NTA) resin. RESULTS The synthetic gene coding APOBEC3G was successfully cloned into the pQE80L vector and could be expressed abundantly in E. coli BL21 in the presence of IPTG. CONCLUSIONS Recombinant APOBEC3G is robustly expressed in E. coli BL21, and the APOBEC3G protein could be purified by using Ni-NTA. The molecular weight of the recombinant APOBEC3G produced is smaller than the expected value. However, the protein is predicted to be able to interact with Vif because this interaction is determined by a specific domain located on the N-terminal of APOBEC3G. Creator An entity primarily responsible for making the resource Rizkyana Avissa, Silvia Tri Widyaningtyas, Budiman Bela Publisher An entity responsible for making the resource available Fakultas Kedokteran Universitas Indonesia Date A point or period of time associated with an event in the lifecycle of the resource 2020-07-02 Contributor An entity responsible for making contributions to the resource Sri Wahyuni Rights Information about rights held in and over the resource ISSN : 0853-1773 Format The file format, physical medium, or dimensions of the resource PDF Language A language of the resource English Type The nature or genre of the resource Text Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020 APOBEC3G codon usage Jurnal Internasional prokaryote gene expression https://repository.horizon.ac.id/files/original/493f0856e5ee54cea5d664fe37fd04cc.pdf 48f6dcb08b0a835ac6bf044867f0a7e1 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Medical Journal of Indonesia Vol. 29 No. 2 2020 FKUI Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020 Neutrophil-to-Lymphocyte Ratio for Predictor of in-Hospital Mortality in ST-Segment Elevation Myocardial Infarction: A Meta-Analysis Subject The topic of the resource in-hospital mortality, neutrophil-to-lymphocyte ratio, STEMI Description An account of the resource BACKGROUND ST-segment elevation myocardial infarction (STEMI) is the most life-threatening condition of acute coronary syndrome that carries a poor prognosis of in-hospital mortality. Multiple scoring systems have been developed to predict in-hospital mortality and other cardiovascular events. Neutrophil-to-lymphocyte ratio (NLR) is hardly used as a predictor of in-hospital mortality. This study was aimed to determine the predictive value of NLR concerning in-hospital mortality in STEMI patients. METHODS Literature search and pooled analysis related to studies on MEDLINE/PubMed, EBSCO, Science Direct, Cochrane, and ProQuest were retrieved. Inclusion criteria were met if they were cohort studies, the subjects were STEMI patient, contained pretreatment NLR cut-off, and considered in-hospital mortality, which is defined as cardiac or all-cause mortality. Quality assessment was conducted using Newcastle-Ottawa scale. Review Manager version 5.3 (The Nordic Cochrane Centre, Copenhagen) was used for meta-analysis. RESULTS We found 12 studies with a total of 7,251 STEMI subjects with median NLR cut�off value of 5.6. Elevated NLR on admission carries a high risk of in-hospital mortality (odds ratio [OR] = 3.00, 95% confidence interval [CI] = 2.46–3.67). A slightly higher risk of all-cause mortality (OR = 2.74, 95% CI = 1.99–3.77) was observed compared with cardiac-related mortality (OR = 3.20, 95% CI = 2.47–4.14). No significant heterogeneity was observed between these studies (p = 0.46, I2 = 0%). CONCLUSIONS Elevated NLR predicts a higher in-hospital mortality rate of STEMI patients. Creator An entity primarily responsible for making the resource Rodry Mikhael, Evan Hindoro, Sharleen Taner, Antonia Anna Lukito Publisher An entity responsible for making the resource available Fakultas Kedokteran Universitas Indonesia Date A point or period of time associated with an event in the lifecycle of the resource 2020-07-02 Contributor An entity responsible for making contributions to the resource Sri Wahyuni Rights Information about rights held in and over the resource ISSN : 0853-1773 Format The file format, physical medium, or dimensions of the resource PDF Language A language of the resource English Type The nature or genre of the resource Text Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020 in-hospital mortality Jurnal Internasional neutrophil-to-lymphocyte ratio STEMI https://repository.horizon.ac.id/files/original/8edcc306470e580492ea74c2b6f35fa1.pdf 57f3973e8b7ea24cd32bf6403b252490 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Medical Journal of Indonesia Vol. 29 No. 2 2020 FKUI Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020 A Case Series of Eight Scoliosis Patients undergone Pedicle Screw Placement with Freehand Technique: Study for Safety and Accuracy Subject The topic of the resource pedicle screws, scoliosis Description An account of the resource BACKGROUND Pedicle screws and rods are routinely inserted in the vertebrae thoracic to lumbar in scoliosis reconstruction surgery to gain deformity correction and fusion. Intraoperative imaging is often needed to ensure accuracy, but surgical time will be longer and there will be more radiation exposure. Meanwhile, freehand technique is accepted as safe. This study was aimed to evaluate the accuracy of screw insertion in the freehand technique used in adolescent scoliosis surgery. METHODS This case series evaluated a total of 127 pedicle screws inserted using the freehand technique in 8 out of 28 adolescent scoliosis patients from 2011 to 2016 whom agreed for computed tomography (CT) scan follow-up from 2011 to 2016 in Dr. Seotomo Hospital, Surabaya. The accuracy and safety of the freehand technique were evaluated postoperatively in each patient using a CT scan. A successful screw was considered accurate if it was within the vertebral body and inside the safety zone (within the limit of 2–4 mm to the medial or lateral side of the vertebral body. RESULTS Of the 127 screws inserted, 106 (83.5%) were accurately placed, and 110 (86.6%) were within the safe zone. According to the level of the spine, in the upper and middle thoracic spine, the inserted screws were accurate in 69.4% and 74.9% within the safety zone, whereas in the lower thoracic and lumbar spine, 94.0% and 95.0% within the safety zone. CONCLUSIONS The freehand technique in scoliosis reconstructive surgery performed by experienced surgeons is accurate and safe. Creator An entity primarily responsible for making the resource Komang Agung Irianto, Marquee Kenny Tumbelaka Publisher An entity responsible for making the resource available Fakultas Kedokteran Universitas Indonesia Date A point or period of time associated with an event in the lifecycle of the resource 2020-07-02 Contributor An entity responsible for making contributions to the resource Sri Wahyuni Rights Information about rights held in and over the resource ISSN : 0853-1773 Format The file format, physical medium, or dimensions of the resource PDF Language A language of the resource English Type The nature or genre of the resource Text Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020 Jurnal Internasional pedicle screws scoliosis https://repository.horizon.ac.id/files/original/e95306edd109d34b4130cbd822a0dac7.jpg 1b4f6c3461357c2ccb3d6eec7b814100 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Medical Journal of Indonesia Vol. 29 No. 2 2020 FKUI Still Image A static visual representation. Examples include paintings, drawings, graphic designs, plans and maps. Recommended best practice is to assign the type Text to images of textual materials. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Cover Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020