Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 30 No. 3 2021
Effect of Hyperglycemia on Fertility in Streptozotocin-Induced Diabetic Male Wistar Rats: Focus on Glucose Transporters and Oxidative Stress
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Title
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 30 No. 3 2021
Effect of Hyperglycemia on Fertility in Streptozotocin-Induced Diabetic Male Wistar Rats: Focus on Glucose Transporters and Oxidative Stress
Effect of Hyperglycemia on Fertility in Streptozotocin-Induced Diabetic Male Wistar Rats: Focus on Glucose Transporters and Oxidative Stress
Subject
GLUT1, GLUT3, glutathione peroxidase, infertility, MCT4, Nrf2
Description
BACKGROUND Glucose transporters (GLUTs) and oxidant metabolism are associated with the mechanism of infertility. This study evaluated the impact of hyperglycemia on glucose and oxidant metabolisms of Sertoli cells (SCs).
METHODS This study was an animal study to investigate the expression of messenger RNA monocarboxylate transporter 4 (MCT4), GLUT1, GLUT3, nuclear factor erythroid 2-related factor 2 (Nrf2), glutathione peroxidase, catalase (CAT), and lactate dehydrogenase A (LDHA) of Wistar rats testes that were induced hyperglycemia. Reverse transcription polymerase chain reaction analysis was used. Hyperglycemic state in the Wistar rats was induced by streptozotocin. 24 rats were divided into 3 groups: non-hyperglycemia (control), 2-week, and 4-week hyperglycemic state. All data were collected and analyzed using SPSS version 15.0 (IBM Corp., USA).
RESULTS The expression of glucose transporter (GLUT1 and GLUT3), lactate transporter (MCT4), and cellular defense protein against oxidant (Nrf2 and CAT) was significantly increased in the 2-week and 4-week hyperglycemic state groups with
p<0.01, respectively.
CONCLUSIONS Hyperglycemic state affects the metabolism of SCs. Alteration of GLUTs and oxidative metabolism may indicate metabolic alterations by a prolonged exposure to hyperglycemia that may be responsible for diabetes-related male infertility.
METHODS This study was an animal study to investigate the expression of messenger RNA monocarboxylate transporter 4 (MCT4), GLUT1, GLUT3, nuclear factor erythroid 2-related factor 2 (Nrf2), glutathione peroxidase, catalase (CAT), and lactate dehydrogenase A (LDHA) of Wistar rats testes that were induced hyperglycemia. Reverse transcription polymerase chain reaction analysis was used. Hyperglycemic state in the Wistar rats was induced by streptozotocin. 24 rats were divided into 3 groups: non-hyperglycemia (control), 2-week, and 4-week hyperglycemic state. All data were collected and analyzed using SPSS version 15.0 (IBM Corp., USA).
RESULTS The expression of glucose transporter (GLUT1 and GLUT3), lactate transporter (MCT4), and cellular defense protein against oxidant (Nrf2 and CAT) was significantly increased in the 2-week and 4-week hyperglycemic state groups with
p<0.01, respectively.
CONCLUSIONS Hyperglycemic state affects the metabolism of SCs. Alteration of GLUTs and oxidative metabolism may indicate metabolic alterations by a prolonged exposure to hyperglycemia that may be responsible for diabetes-related male infertility.
Creator
Sakti Ronggowardhana Brodjonegoro, Tanaya Ghinorawa, Nickanor Kaladius Reumy Wonatorey, Andy Zulfiqqar, Didik Setyo Heriyanto
Publisher
Fakultas Kedokteran Universitas Indonesia
Date
2021-10-12
Contributor
Sri Wahyuni
Rights
ISSN : 0853-1773
Format
PDF
Language
English
Type
Text
Coverage
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 30 No. 3 2021
Files
Citation
Sakti Ronggowardhana Brodjonegoro, Tanaya Ghinorawa, Nickanor Kaladius Reumy Wonatorey, Andy Zulfiqqar, Didik Setyo Heriyanto, “Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 30 No. 3 2021
Effect of Hyperglycemia on Fertility in Streptozotocin-Induced Diabetic Male Wistar Rats: Focus on Glucose Transporters and Oxidative Stress,” Repository Horizon University Indonesia, accessed November 21, 2024, https://repository.horizon.ac.id/items/show/1008.
Effect of Hyperglycemia on Fertility in Streptozotocin-Induced Diabetic Male Wistar Rats: Focus on Glucose Transporters and Oxidative Stress,” Repository Horizon University Indonesia, accessed November 21, 2024, https://repository.horizon.ac.id/items/show/1008.