Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 30 No. 3 2021
Multi-Hole Spherical CT Scan Method to Characterize Large Quantities of Bones in Rats
Dublin Core
Title
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 30 No. 3 2021
Multi-Hole Spherical CT Scan Method to Characterize Large Quantities of Bones in Rats
Multi-Hole Spherical CT Scan Method to Characterize Large Quantities of Bones in Rats
Subject
bone quality, CT scan, multi-hole spherical model, osteoporosis, ovariectomy
Description
BACKGROUND New therapeutic options are often explored in in vivo studies using animals like rats. Since rats are small, it is difficult to examine them in a computed tomography (CT) scan. This study aimed to introduce a multi-hole spherical model CT scan method as a new, fast, economical, and reliable method to characterize large quantities of rat bones at once in estimating the timing of osteoporosis in
ovariectomized white rats.
METHODS 50 female white rats (12 weeks old) were treated as the control group, and 40 rats of the same age were ovariectomized to establish the osteoporosis model. Sham rats were sacrificed at 13, 15, 17, 19, and 21 weeks old, while the ovariectomized rats were sacrificed at 15, 17, 19, and 21 weeks old. Afterward, tibia bones were removed, placed in the multi-hole spherical model, and characterized using a CT scan. Their characteristics were compared using a scanning electron microscope (SEM), transmission electron
microscopy (TEM), and X-ray diffraction (XRD).
RESULTS The Hounsfield unit scores resulted from the multi-hole spherical model CT scan method of tibia bones of rats were consistent with the percentage of the osteocyte cavities, canalicular diameters, and crystal size. The multi-hole spherical model CT scan method could produce 50 times more data than the SEM, TEM, or XRD.
CONCLUSIONS Multi-hole spherical model CT scan was considered good and reliable in assessing bone quality parameters in rat samples simultaneously.
ovariectomized white rats.
METHODS 50 female white rats (12 weeks old) were treated as the control group, and 40 rats of the same age were ovariectomized to establish the osteoporosis model. Sham rats were sacrificed at 13, 15, 17, 19, and 21 weeks old, while the ovariectomized rats were sacrificed at 15, 17, 19, and 21 weeks old. Afterward, tibia bones were removed, placed in the multi-hole spherical model, and characterized using a CT scan. Their characteristics were compared using a scanning electron microscope (SEM), transmission electron
microscopy (TEM), and X-ray diffraction (XRD).
RESULTS The Hounsfield unit scores resulted from the multi-hole spherical model CT scan method of tibia bones of rats were consistent with the percentage of the osteocyte cavities, canalicular diameters, and crystal size. The multi-hole spherical model CT scan method could produce 50 times more data than the SEM, TEM, or XRD.
CONCLUSIONS Multi-hole spherical model CT scan was considered good and reliable in assessing bone quality parameters in rat samples simultaneously.
Creator
Neng Nenden Mulyaningsih, Ariadne Lakshmidevi Juwono, Djarwani Soeharso Soejoko, Dewi Apri Astuti
Publisher
Fakultas Kedokteran Universitas Indonesia
Date
2021-10-12
Contributor
Sri Wahyuni
Rights
ISSN : 0853-1773
Format
PDF
Language
English
Type
Text
Coverage
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 30 No. 3 2021
Files
Citation
Neng Nenden Mulyaningsih, Ariadne Lakshmidevi Juwono, Djarwani Soeharso Soejoko, Dewi Apri Astuti, “Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 30 No. 3 2021
Multi-Hole Spherical CT Scan Method to Characterize Large Quantities of Bones in Rats,” Repository Horizon University Indonesia, accessed March 12, 2025, https://repository.horizon.ac.id/items/show/1012.
Multi-Hole Spherical CT Scan Method to Characterize Large Quantities of Bones in Rats,” Repository Horizon University Indonesia, accessed March 12, 2025, https://repository.horizon.ac.id/items/show/1012.