Nanocurcumin Innovation as an Anti-Apoptosis of
Ovarian Granulosa Cells in White Rats Exposed to
Lead Acetate (PbAc)

Nanocurcumin Innovation as an Anti-Apoptosis of
Ovarian Granulosa Cells in White Rats Exposed to
Lead Acetate (PbAc)

innovation,

nanocurcumin, anti-
apoptosis, PbAc

Introduction: Exposure to Pb causes increased apoptosis of ovarian
granulosa cells through oxidative stress mechanism. Curcumin has
protective effects on reproductive organs, anti-apoptotic, antioxidant in
normal cells. Curcumin in innovated nano form can function as an effective
anti-apoptosis in ovarian granulosa cells of rats due to PbAc exposure.
Methods: Thirty female rats were divided into 3 groups, the negative
control group (the rats receiving distilled water, in each 90 minutes
receiving corn oil), positive control group (the rats receiving PbAc of 30
mg/kg BW, in each 90 minutes receiving corn oil), the experimental group,
in which the rats receiving PbAc of 30 mg/kg BW, and in each 90 minutes
receiving nanocurcumin of 200 mg/kg BW. All groups received treatment
orally once a day for 30 days. On day 31 the rats to granulosa cell apoptosis
examination using Tunnel method.
Results: Rate of apoptosis was in the positive control group (5.4 ±
0.8%/micro) and the lowest was in the experimental group (1.1 ±
0.5%/micro) and the negative control group (1.2 ± 0.6). The experimental
group showed the same p value as the negative control group (p = .095)
and different p value (p = .010) from the positive control group. These
findings indicated that the innovation of curcumin in nano form at a dose
of 200 mg/KgBW reduced apoptosis of rat ovarian granulosa cells due to
PbAc exposure.
Conclusion: The innovation of curcumin in nano form has the potential as
an effective natural anti-apoptosis in rats ovarian granulosa cells exposed
to PbAc.

Anis Satus Syarifah1*

https://doi.org/10.37363/bnr.2022.3279

23 July 2022

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