Safety and Health at Work Vol. 13 Supplement 1 2022
Differential effects of 1-MHz low intensity pulsed ultrasound in human keratinocytes
Dublin Core
Title
Safety and Health at Work Vol. 13 Supplement 1 2022
Differential effects of 1-MHz low intensity pulsed ultrasound in human keratinocytes
Differential effects of 1-MHz low intensity pulsed ultrasound in human keratinocytes
Subject
1-MHz low intensity pulsed ultrasound in human keratinocytes
Description
Introduction: The wide use of low intensity pulsed ultrasound (LIPUS) suggests the need to better understand the related biological effects. We investigated the mechanical stress produced by 1 MHz LIPUS in human keratinocytes in terms of deregulation of the inflammatory cytokines IL-6, cell viability and membrane permeability. DNA damage was also tested to evaluate clastogenic and aneugenic LIPUS-induced genomic damage.
Materials and Methods: In vitro exposure at 1 MHz LIPUS (10% duty
cycle and pulses of 300 s) were performed on HaCaT cell line at varying Intensity (Ispta <100 mW/cm2) and exposure time up to 1 hour. Pro-inflammatory cytokines and activation of nuclear factor-B (NF-
B) were detected by real-time qPCR, ELISA and immunofluorescence
assays. Related effects on plasma membrane, cell cycle distribution
and apoptosis were also evaluated through confocal microscopy,
flow cytometry and by following the gene expression of Bax/Bcl-
2. Finally, cytokinesis-blocked BN cell preparations and CREST
immunofluorescence staining were performed.
Results: Our results pointed out that 1 hour exposure of 1 MHz
LIPUS (Ispta 65 mW/cm2) promotes both significant IL-6 gene overexpression and protein secretion which is associated with the
activation of NF-B. We also found concomitant effects on membrane
permeability and cell viability. Micronucleus test evidenced no
clastogenic damage at any dose tested and a significant induction of
aneugenic damage only with the highest dose employed probably
due to the perturbation of the mitotic apparatus.
Acknowledgement: This research was realized with the financial
support of INAIL under the agreement BRIC2019
Materials and Methods: In vitro exposure at 1 MHz LIPUS (10% duty
cycle and pulses of 300 s) were performed on HaCaT cell line at varying Intensity (Ispta <100 mW/cm2) and exposure time up to 1 hour. Pro-inflammatory cytokines and activation of nuclear factor-B (NF-
B) were detected by real-time qPCR, ELISA and immunofluorescence
assays. Related effects on plasma membrane, cell cycle distribution
and apoptosis were also evaluated through confocal microscopy,
flow cytometry and by following the gene expression of Bax/Bcl-
2. Finally, cytokinesis-blocked BN cell preparations and CREST
immunofluorescence staining were performed.
Results: Our results pointed out that 1 hour exposure of 1 MHz
LIPUS (Ispta 65 mW/cm2) promotes both significant IL-6 gene overexpression and protein secretion which is associated with the
activation of NF-B. We also found concomitant effects on membrane
permeability and cell viability. Micronucleus test evidenced no
clastogenic damage at any dose tested and a significant induction of
aneugenic damage only with the highest dose employed probably
due to the perturbation of the mitotic apparatus.
Acknowledgement: This research was realized with the financial
support of INAIL under the agreement BRIC2019
Creator
Fabio Domenici, Sabrina Giantulli, Elisabetta Tortorella, Damiano Palmieri, Martina Piermarini, Ion Udroiu, Raffaele Palomba, Claudia Giliberti, Stefania Morrone, Susanna Scarpa, Chiara Di Nunzio, Gaio Paradossi, Antonella Sgura, Angelico Bedini, Ida Silvestri
Publisher
Elsevier Korea LLC
Date
January 2022
Contributor
Sri Wahyuni
Format
PDF
Language
English
Type
Text
Coverage
Safety and Health at Work Vol. 13 Supplement 1 2022
Files
Citation
Fabio Domenici, Sabrina Giantulli, Elisabetta Tortorella, Damiano Palmieri, Martina Piermarini, Ion Udroiu, Raffaele Palomba, Claudia Giliberti, Stefania Morrone, Susanna Scarpa, Chiara Di Nunzio, Gaio Paradossi, Antonella Sgura, Angelico Bedini, Ida Silvestri, “Safety and Health at Work Vol. 13 Supplement 1 2022
Differential effects of 1-MHz low intensity pulsed ultrasound in human keratinocytes,” Repository Horizon University Indonesia, accessed April 10, 2025, https://repository.horizon.ac.id/items/show/2350.
Differential effects of 1-MHz low intensity pulsed ultrasound in human keratinocytes,” Repository Horizon University Indonesia, accessed April 10, 2025, https://repository.horizon.ac.id/items/show/2350.