Safety and Health at Work Vol. 13 Supplement 2022
Asbestos-caused key events of altered expression of gene in mesothelioma patients
Dublin Core
Title
Safety and Health at Work Vol. 13 Supplement 2022
Asbestos-caused key events of altered expression of gene in mesothelioma patients
Asbestos-caused key events of altered expression of gene in mesothelioma patients
Subject
Asbestos, key events of altered expression of gene, mesothelioma patients
Description
Introduction: Asbestos-related disease is a global problem, where malignant mesothelioma is characteristic. Our previous studies clarified that asbestos exposure causes altered characteristics in immune cells, some of which were also confirmed in mesothelioma patients. Additionally, our recent study found that long-term exposure to chrysotile asbestos resulted in low cytotoxicity and INF-? production by CD8+ T cell line. Therefore, we examined alteration in gene expression caused by asbestos by using transcriptome analysis.
Materials and Methods: EBT-8 of human CD8+ T cell line was cultured under exposure to chrysotile A, chrysotile JAWE, crocido- lite asbestos or TiO2 particle at 30 mg/ml compared with the control. Cells were analyzed weekly for mRNAs including IFN-? by RT-qPCR. Finally, RNA-sequencing was conducted for total RNA of cell samples (n1⁄43, 5 groups).
Results: Regardless of asbestos types, the cells showed gradual decline in IFN-? expression. Transcriptome analyses showed that all of the asbestos types share decreased and increased expression of limited genes, in which over 4-fold changes were shown in 5 transcripts including IFN-?. However, chrysotile A and JAWE exposures showed positive correlation in all of the altered gene expressions, whereas crocidolite showed relatively low effect and negative correlation with chrysotile A in a part of the genes.
Conclusions: These results indicate that continuous exposure to asbestos causes a key event in T lymphocytes served by limited genes leading to immune suppression, where chrysotile and crocidolite showed different impacts, related with mesothelioma.
Materials and Methods: EBT-8 of human CD8+ T cell line was cultured under exposure to chrysotile A, chrysotile JAWE, crocido- lite asbestos or TiO2 particle at 30 mg/ml compared with the control. Cells were analyzed weekly for mRNAs including IFN-? by RT-qPCR. Finally, RNA-sequencing was conducted for total RNA of cell samples (n1⁄43, 5 groups).
Results: Regardless of asbestos types, the cells showed gradual decline in IFN-? expression. Transcriptome analyses showed that all of the asbestos types share decreased and increased expression of limited genes, in which over 4-fold changes were shown in 5 transcripts including IFN-?. However, chrysotile A and JAWE exposures showed positive correlation in all of the altered gene expressions, whereas crocidolite showed relatively low effect and negative correlation with chrysotile A in a part of the genes.
Conclusions: These results indicate that continuous exposure to asbestos causes a key event in T lymphocytes served by limited genes leading to immune suppression, where chrysotile and crocidolite showed different impacts, related with mesothelioma.
Creator
Yasumitsu Nishimura
Publisher
Elsevier Korea LLC
Date
January 2022
Contributor
Sri Wahyuni
Format
PDF
Language
English
Type
Text
Coverage
Safety and Health at Work Vol. 13 Supplement 2022
Files
Citation
Yasumitsu Nishimura, “Safety and Health at Work Vol. 13 Supplement 2022
Asbestos-caused key events of altered expression of gene in mesothelioma patients,” Repository Horizon University Indonesia, accessed November 21, 2024, https://repository.horizon.ac.id/items/show/2392.
Asbestos-caused key events of altered expression of gene in mesothelioma patients,” Repository Horizon University Indonesia, accessed November 21, 2024, https://repository.horizon.ac.id/items/show/2392.