PROSIDING INTERNASIONAL KEPERAWATAN Proceedings of the International Conference on Nursing and Health Sciences Volume 1 No 1, November 2020
UPREGULATION OF MRNA TNF-α IN SKELETAL MUSCLE TISSUE OF STREPTOZOTOCIN-INDUCED DIABETIC RAT
Dublin Core
Title
PROSIDING INTERNASIONAL KEPERAWATAN Proceedings of the International Conference on Nursing and Health Sciences Volume 1 No 1, November 2020
UPREGULATION OF MRNA TNF-α IN SKELETAL MUSCLE TISSUE OF STREPTOZOTOCIN-INDUCED DIABETIC RAT
UPREGULATION OF MRNA TNF-α IN SKELETAL MUSCLE TISSUE OF STREPTOZOTOCIN-INDUCED DIABETIC RAT
Subject
diabetes mellitus; TNF-α; inflammation; skeletal muscle
Description
Inflammation is a molecular mechanism that linking obesity and ageing with insulin resistance in type 2 diabetes
mellitus (DM). Although type 1 DM is primarily caused by insulin deficiency, but insulin resistance also
prominent feature in this disease. It is not fully understood whether inflammation also contribute in insulin
resistance phenotype in type 1 DM. This study aimed to assess mRNA TNF-α expression in the skeletal muscle
tissue of type 1 diabetes mellitus rat model.This in-vivo study used 18 adults male wistar rats. The study
conducted at Department of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah
Mada from July to October 2020. Male Wistar rats divided into control group (CDM, n=6), and diabetes mellitus
group which is divided into 1-month DM group (DM1M, n=6), and 2-month DM group (DM2M, n=6). The DM
model was conducted through single intraperitoneal injection of Streptozotocin 60 mg/kg Body Weight (BW). At
the end of study, rats were sacrificed and the gastrocnemius muscle was harvested. The expression of mRNA
TNF-α was measured by RT-PCR. Statistical analysis was conducted using One Way ANOVA test. Blood
glucose level were significantly higher in DM groups compared to control group (p<0.05). The body weight of
DM groups was significantly lower after 1 month and 2 months DM period compared to control group (p<0.05).
DM groups demonstrated upregulation of mRNA TNF-α compared to control group (p<0.05). Type 1 diabetes
mellitus model demonstrated upregulation of mRNA TNF-α in skeletal muscle tissue.
mellitus (DM). Although type 1 DM is primarily caused by insulin deficiency, but insulin resistance also
prominent feature in this disease. It is not fully understood whether inflammation also contribute in insulin
resistance phenotype in type 1 DM. This study aimed to assess mRNA TNF-α expression in the skeletal muscle
tissue of type 1 diabetes mellitus rat model.This in-vivo study used 18 adults male wistar rats. The study
conducted at Department of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah
Mada from July to October 2020. Male Wistar rats divided into control group (CDM, n=6), and diabetes mellitus
group which is divided into 1-month DM group (DM1M, n=6), and 2-month DM group (DM2M, n=6). The DM
model was conducted through single intraperitoneal injection of Streptozotocin 60 mg/kg Body Weight (BW). At
the end of study, rats were sacrificed and the gastrocnemius muscle was harvested. The expression of mRNA
TNF-α was measured by RT-PCR. Statistical analysis was conducted using One Way ANOVA test. Blood
glucose level were significantly higher in DM groups compared to control group (p<0.05). The body weight of
DM groups was significantly lower after 1 month and 2 months DM period compared to control group (p<0.05).
DM groups demonstrated upregulation of mRNA TNF-α compared to control group (p<0.05). Type 1 diabetes
mellitus model demonstrated upregulation of mRNA TNF-α in skeletal muscle tissue.
Creator
Akbar Satria Fitriawan, Christin Wiyani, Endang Nurul Syafitri, Ririn Wahyu Widayati
Source
http://jurnal.globalhealthsciencegroup.com/index.php/PICNHS
Publisher
Global Health Science Group
Date
November 2020
Contributor
PERI IRAWAN
Format
PDF
Language
ENGLISH
Type
TEXT
Files
Citation
Akbar Satria Fitriawan, Christin Wiyani, Endang Nurul Syafitri, Ririn Wahyu Widayati, “PROSIDING INTERNASIONAL KEPERAWATAN Proceedings of the International Conference on Nursing and Health Sciences Volume 1 No 1, November 2020
UPREGULATION OF MRNA TNF-α IN SKELETAL MUSCLE TISSUE OF STREPTOZOTOCIN-INDUCED DIABETIC RAT,” Repository Horizon University Indonesia, accessed December 26, 2024, https://repository.horizon.ac.id/items/show/2992.
UPREGULATION OF MRNA TNF-α IN SKELETAL MUSCLE TISSUE OF STREPTOZOTOCIN-INDUCED DIABETIC RAT,” Repository Horizon University Indonesia, accessed December 26, 2024, https://repository.horizon.ac.id/items/show/2992.