Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020
Cytotoxic Effect of Natural Cuttlefish Bone Xenograft: An in Vitro and in Vivo Study
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Title
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020
Cytotoxic Effect of Natural Cuttlefish Bone Xenograft: An in Vitro and in Vivo Study
Cytotoxic Effect of Natural Cuttlefish Bone Xenograft: An in Vitro and in Vivo Study
Subject
bone substitute, cuttlefish bone, cytotoxicity tests, xenograft
Description
BACKGROUND Commercialized synthetic bone grafts are commonly used to replace the bone defect. Cuttlefish bone is naturally available and widely studied, but the specific cytotoxicity test has not been conducted. This study aimed to evaluate the cytotoxicity of the xenograft compared to commercial grafts.
METHODS We performed an in vitro test evaluating the viability of human mesenchymal stem cells (hMSCs) when cultured for 48 hours with the tested materials (cuttlefish bone graft and fabricated PerOssal®). The trypsinized itochondrial activity of the viable hMSC was assayed based on colorimetry of the formazan color change. The
tested material was considered nontoxic if >70% of the hMSCs were viable. The in vivo cytotoxic effect was evaluated by implanting the graft material in the femoral muscle of New Zealand (NZ) white rabbits. Nine rabbits were used in each test (cuttlefish bone, PerOssal®, and NaCl 0.9%). The systemic acute pyrogenic effect was evaluated
based on 72 hours body weight changes and rectal temperature changes every 30 min in the first 3 hours and 72 hours post-implantation.
RESULTS The mean percentage of hMSC viability when cultured with cuttlefish bone graft and PerOssal® was comparable (93.47% and 105.37%, respectively, p = 0.240). The in vivo cytotoxicity on NZ rabbit was similar between all tested materials, as shown by the minor changes in body weight (<10% body weight, p = 0.391) and rectal temperature (<0.5°C, p = 0.127).
CONCLUSIONS Cuttlefish bone xenograft and fabricated PerOssal® have a similar noncytotoxic effects on hMSCs and non-pyrogenic systemic effects on rabbits.
METHODS We performed an in vitro test evaluating the viability of human mesenchymal stem cells (hMSCs) when cultured for 48 hours with the tested materials (cuttlefish bone graft and fabricated PerOssal®). The trypsinized itochondrial activity of the viable hMSC was assayed based on colorimetry of the formazan color change. The
tested material was considered nontoxic if >70% of the hMSCs were viable. The in vivo cytotoxic effect was evaluated by implanting the graft material in the femoral muscle of New Zealand (NZ) white rabbits. Nine rabbits were used in each test (cuttlefish bone, PerOssal®, and NaCl 0.9%). The systemic acute pyrogenic effect was evaluated
based on 72 hours body weight changes and rectal temperature changes every 30 min in the first 3 hours and 72 hours post-implantation.
RESULTS The mean percentage of hMSC viability when cultured with cuttlefish bone graft and PerOssal® was comparable (93.47% and 105.37%, respectively, p = 0.240). The in vivo cytotoxicity on NZ rabbit was similar between all tested materials, as shown by the minor changes in body weight (<10% body weight, p = 0.391) and rectal temperature (<0.5°C, p = 0.127).
CONCLUSIONS Cuttlefish bone xenograft and fabricated PerOssal® have a similar noncytotoxic effects on hMSCs and non-pyrogenic systemic effects on rabbits.
Creator
Komang Agung Irianto, Suyenci Limbong
Publisher
Fakultas Kedokteran Universitas Indonesia
Date
2020-07-02
Contributor
Sri Wahyuni
Rights
ISSN : 0853-1773
Format
PDF
Language
English
Type
Text
Coverage
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020
Files
Citation
Komang Agung Irianto, Suyenci Limbong, “Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020
Cytotoxic Effect of Natural Cuttlefish Bone Xenograft: An in Vitro and in Vivo Study,” Repository Horizon University Indonesia, accessed November 21, 2024, https://repository.horizon.ac.id/items/show/857.
Cytotoxic Effect of Natural Cuttlefish Bone Xenograft: An in Vitro and in Vivo Study,” Repository Horizon University Indonesia, accessed November 21, 2024, https://repository.horizon.ac.id/items/show/857.