Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 30 No. 1 2021
Efficacy of Adjuvant Intrastromal and Combination of Intrastromal and Intracameral Voriconazole in Aspergillus Fumigatus-Induced Moderate Fungal Keratitis in Rabbits
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Title
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 30 No. 1 2021
Efficacy of Adjuvant Intrastromal and Combination of Intrastromal and Intracameral Voriconazole in Aspergillus Fumigatus-Induced Moderate Fungal Keratitis in Rabbits
Efficacy of Adjuvant Intrastromal and Combination of Intrastromal and Intracameral Voriconazole in Aspergillus Fumigatus-Induced Moderate Fungal Keratitis in Rabbits
Subject
Aspergillus fumigatus, fungal keratitis, intrastromal, voriconazole
Description
BACKGROUND There is no in vivo evidence for the effectiveness of adjuvant intrastromal and combination of intrastromal and intracameral voriconazole (VCZ) for treating Aspergillus fumigatus keratitis. This study aimed to compare the efficacy of both agents against it.
METHODS A randomized, masked, controlled experimental study was conducted on 11 albino New Zealand white rabbits in which moderate fungal keratitis was induced by inoculating spores of A. fumigatus to the cornea. The rabbits were allocated into 3 groups: 50 μg/0.1 ml intrastromal VCZ injection, 50 μg/0.1 ml intrastromal and
intracameral VCZ injections, and topical VCZ (control). The treatment was given 5 days after inoculation. Epithelial defect, infiltrate size, corneal ulcer depth, and hypopyon were evaluated clinically. Histopathological and mycological examinations were also done 14 days after treatment.
RESULTS All rabbits in the adjuvant treatment groups demonstrated a tendency of a better clinical response with decreasing size of epithelial defect (p = 0.679) and infiltrate (p = 0.755) than in the control group. Direct microscopy, corneal culture, and chop corneal tissue culture were still positive in most of the rabbits from all groups. Histopathological examination showed an increase of inflammatory cells after treatment in all groups, especially in rabbits which were inoculated with A. fumigatus
spores in both eyes.
CONCLUSIONS An adjuvant combination of intrastromal and intracameral VCZ showed a tendency of better clinical response for A. fumigatus-induced moderate fungal keratitis in rabbits.
METHODS A randomized, masked, controlled experimental study was conducted on 11 albino New Zealand white rabbits in which moderate fungal keratitis was induced by inoculating spores of A. fumigatus to the cornea. The rabbits were allocated into 3 groups: 50 μg/0.1 ml intrastromal VCZ injection, 50 μg/0.1 ml intrastromal and
intracameral VCZ injections, and topical VCZ (control). The treatment was given 5 days after inoculation. Epithelial defect, infiltrate size, corneal ulcer depth, and hypopyon were evaluated clinically. Histopathological and mycological examinations were also done 14 days after treatment.
RESULTS All rabbits in the adjuvant treatment groups demonstrated a tendency of a better clinical response with decreasing size of epithelial defect (p = 0.679) and infiltrate (p = 0.755) than in the control group. Direct microscopy, corneal culture, and chop corneal tissue culture were still positive in most of the rabbits from all groups. Histopathological examination showed an increase of inflammatory cells after treatment in all groups, especially in rabbits which were inoculated with A. fumigatus
spores in both eyes.
CONCLUSIONS An adjuvant combination of intrastromal and intracameral VCZ showed a tendency of better clinical response for A. fumigatus-induced moderate fungal keratitis in rabbits.
Creator
Dyah Tjintya Sarika, Melva Louisa, Anna Rozaliyani, Evelina, Made Susiyanti
Publisher
Fakultas Kedokteran Universitas Indonesia
Date
2021-03-31
Contributor
Sri Wahyuni
Rights
ISSN : 0853-1773
Format
PDF
Language
English
Type
Text
Coverage
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 30 No. 1 2021
Files
Citation
Dyah Tjintya Sarika, Melva Louisa, Anna Rozaliyani, Evelina, Made Susiyanti
, “Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 30 No. 1 2021
Efficacy of Adjuvant Intrastromal and Combination of Intrastromal and Intracameral Voriconazole in Aspergillus Fumigatus-Induced Moderate Fungal Keratitis in Rabbits,” Repository Horizon University Indonesia, accessed October 14, 2024, https://repository.horizon.ac.id/items/show/957.
Efficacy of Adjuvant Intrastromal and Combination of Intrastromal and Intracameral Voriconazole in Aspergillus Fumigatus-Induced Moderate Fungal Keratitis in Rabbits,” Repository Horizon University Indonesia, accessed October 14, 2024, https://repository.horizon.ac.id/items/show/957.