Medical Journal of Indonesia Vol. 31 No. 1 2022 FKUI
Basic Medical Research Effect of Immediate Electrical Stimulation in the Distal Segment of the Nerve with Wallerian Degeneration in Rats with Sciatic Nerve Injury
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Title
Medical Journal of Indonesia Vol. 31 No. 1 2022 FKUI
Basic Medical Research Effect of Immediate Electrical Stimulation in the Distal Segment of the Nerve with Wallerian Degeneration in Rats with Sciatic Nerve Injury
Basic Medical Research Effect of Immediate Electrical Stimulation in the Distal Segment of the Nerve with Wallerian Degeneration in Rats with Sciatic Nerve Injury
Subject
electrical stimulation, sciatic nerve, Wallerian degeneration
Description
ABSTRACT
BACKGROUND Electrical stimulation in the proximal segment is one of the modalities
for peripheral nerve injury, although it is prone to cause excessive axonal sprouting
growth in the proximal segment of the nerve. This study aimed to show that
immediate electrical stimulation in the distal segment of the sciatic nerve in Wistar rats
accelerated Wallerian degeneration by increasing the expression of tumor necrosis
factor-alpha (TNF-α), interleukin (IL)-10, and galectin-3/MAC-2 macrophages to avoid
sprouting axons excessively in the proximal segment.
METHODS This was an experimental study using male Wistar rats (Rattus norvegicus)
with a randomized post-test only control group design. The treatment group received
immediate electrical stimulation (20 Hz, 2 mA, for 5 sec) to the distal nerve after
sciatic nerve injury, while the control group received no treatment. After 3 days, tissue
samples were extracted from the distal segment of the sciatic nerve to examine the
level of TNF-α, IL-10, and galectin 3/Mac-2 macrophages using ELISA and from proximal
nerves to histologically examine the sprouting axons.
RESULTS Rats in the treatment group had higher TNF-α (52.1 [10.32] versus 40.4 [17.71]
pg/100 mg, p = 0.031) and higher IL-10 (918 [167.6] versus 759 [158.9] pg/ml, p = 0.010).
Expression of galectin 3/Mac-2 macrophages was similar in both groups (465 [49.5]
versus 444 [54.4] pg/100 mg, p = 0.247). The number of sprouting axons was lower in
the treatment group (2 [IQR 1–2] versus 2.5 [IQR 2–3], p = 0.003).
CONCLUSIONS Immediate electrical stimulation in the distal segment of the sciatic
nerve can accelerate nerve regeneration.
KEYWORDS electrical stimulation, sciatic nerve, Wallerian degeneration
BACKGROUND Electrical stimulation in the proximal segment is one of the modalities
for peripheral nerve injury, although it is prone to cause excessive axonal sprouting
growth in the proximal segment of the nerve. This study aimed to show that
immediate electrical stimulation in the distal segment of the sciatic nerve in Wistar rats
accelerated Wallerian degeneration by increasing the expression of tumor necrosis
factor-alpha (TNF-α), interleukin (IL)-10, and galectin-3/MAC-2 macrophages to avoid
sprouting axons excessively in the proximal segment.
METHODS This was an experimental study using male Wistar rats (Rattus norvegicus)
with a randomized post-test only control group design. The treatment group received
immediate electrical stimulation (20 Hz, 2 mA, for 5 sec) to the distal nerve after
sciatic nerve injury, while the control group received no treatment. After 3 days, tissue
samples were extracted from the distal segment of the sciatic nerve to examine the
level of TNF-α, IL-10, and galectin 3/Mac-2 macrophages using ELISA and from proximal
nerves to histologically examine the sprouting axons.
RESULTS Rats in the treatment group had higher TNF-α (52.1 [10.32] versus 40.4 [17.71]
pg/100 mg, p = 0.031) and higher IL-10 (918 [167.6] versus 759 [158.9] pg/ml, p = 0.010).
Expression of galectin 3/Mac-2 macrophages was similar in both groups (465 [49.5]
versus 444 [54.4] pg/100 mg, p = 0.247). The number of sprouting axons was lower in
the treatment group (2 [IQR 1–2] versus 2.5 [IQR 2–3], p = 0.003).
CONCLUSIONS Immediate electrical stimulation in the distal segment of the sciatic
nerve can accelerate nerve regeneration.
KEYWORDS electrical stimulation, sciatic nerve, Wallerian degeneration
Creator
Agus Roy Rusly Hariantana Hamid, Sri Maliawan, Dewa Putu Gde Purwa Samatra, Nyoman Mantik Astawa, I Made Bakta, I Made Jawi, Ida Bagus Putra Manuaba, I Made Dewa Sukrama, David Sontani Perdanakusuma
Contributor
peri irawan
Format
pdf
Language
english
Type
text
Files
Citation
Agus Roy Rusly Hariantana Hamid, Sri Maliawan, Dewa Putu Gde Purwa Samatra, Nyoman Mantik Astawa, I Made Bakta, I Made Jawi, Ida Bagus Putra Manuaba, I Made Dewa Sukrama, David Sontani Perdanakusuma, “Medical Journal of Indonesia Vol. 31 No. 1 2022 FKUI
Basic Medical Research Effect of Immediate Electrical Stimulation in the Distal Segment of the Nerve with Wallerian Degeneration in Rats with Sciatic Nerve Injury,” Repository Horizon University Indonesia, accessed December 21, 2024, https://repository.horizon.ac.id/items/show/1038.
Basic Medical Research Effect of Immediate Electrical Stimulation in the Distal Segment of the Nerve with Wallerian Degeneration in Rats with Sciatic Nerve Injury,” Repository Horizon University Indonesia, accessed December 21, 2024, https://repository.horizon.ac.id/items/show/1038.