Medical Journal of Indonesia Vol. 31 No. 2 2022 FKUI
Basic Medical Research Novel and Simple Method using Cable Ties to Induce Intestinal Strangulation in A Rat Model
Dublin Core
Title
Medical Journal of Indonesia Vol. 31 No. 2 2022 FKUI
Basic Medical Research Novel and Simple Method using Cable Ties to Induce Intestinal Strangulation in A Rat Model
Basic Medical Research Novel and Simple Method using Cable Ties to Induce Intestinal Strangulation in A Rat Model
Subject
collagen, fibrosis, intestinal obstruction
Description
BACKGROUND The pathogenesis of intestinal fibrosis after strangulation in abdominal
surgery is not fully understood. Developing an experimental and reliable method is
needed to overcome this problem. This study aimed to develop an effective method for
intestinal strangulation leading to fibrosis, which might induce intestinal obstruction.
METHODS This study was conducted from 2018 to 2019 at the Department of
Pharmacology, Faculty of Medicine, Universitas Indonesia. A total of 24 Sprague
Dawley rats were used in this study. Artificial intestinal strangulation using cable ties
made of durable plastic, which is generally used to ligate objects, was applied, and
rats were further classified into control, 6-hour, and 24-hour strangulation groups.
At the end of the study, the rats were sacrificed, intestinal tissues were collected,
and histomorphological changes were observed using hematoxylin and eosin stain.
Moreover, Masson’s trichome staining was used to assess collagen density.
RESULTS Median collagen density score of the 24-hour strangulation group was higher
than the control. A significant difference in collagen density was found between the
submucosal layer of the 24-hour strangulation group and the control (45.4 [11.4] versus
32.4 [14.0], p<0.001) and between the mucosa of the 6- and 24-hour strangulation
groups and the control (26.9 [17] versus 6.46 [4.3], p = 0.01 and 24.9 [8] versus 6.46
[4.3], p = 0.004, respectively).
CONCLUSIONS The simple use of cable ties adequately promotes intestinal
strangulation.
surgery is not fully understood. Developing an experimental and reliable method is
needed to overcome this problem. This study aimed to develop an effective method for
intestinal strangulation leading to fibrosis, which might induce intestinal obstruction.
METHODS This study was conducted from 2018 to 2019 at the Department of
Pharmacology, Faculty of Medicine, Universitas Indonesia. A total of 24 Sprague
Dawley rats were used in this study. Artificial intestinal strangulation using cable ties
made of durable plastic, which is generally used to ligate objects, was applied, and
rats were further classified into control, 6-hour, and 24-hour strangulation groups.
At the end of the study, the rats were sacrificed, intestinal tissues were collected,
and histomorphological changes were observed using hematoxylin and eosin stain.
Moreover, Masson’s trichome staining was used to assess collagen density.
RESULTS Median collagen density score of the 24-hour strangulation group was higher
than the control. A significant difference in collagen density was found between the
submucosal layer of the 24-hour strangulation group and the control (45.4 [11.4] versus
32.4 [14.0], p<0.001) and between the mucosa of the 6- and 24-hour strangulation
groups and the control (26.9 [17] versus 6.46 [4.3], p = 0.01 and 24.9 [8] versus 6.46
[4.3], p = 0.004, respectively).
CONCLUSIONS The simple use of cable ties adequately promotes intestinal
strangulation.
Creator
Agi Satria Putranto, Fransiscus Dhyanagiri Suyatna, Vivian Soetikno, Yefta Moenadjat
Source
https://doi.org/10.13181/mji.oa.225799
Date
April 14, 2022
Contributor
peri irawan
Format
pdf
Language
english
Type
text
Files
Citation
Agi Satria Putranto, Fransiscus Dhyanagiri Suyatna, Vivian Soetikno, Yefta Moenadjat, “Medical Journal of Indonesia Vol. 31 No. 2 2022 FKUI
Basic Medical Research Novel and Simple Method using Cable Ties to Induce Intestinal Strangulation in A Rat Model,” Repository Horizon University Indonesia, accessed October 31, 2025, https://repository.horizon.ac.id/items/show/1057.
Basic Medical Research Novel and Simple Method using Cable Ties to Induce Intestinal Strangulation in A Rat Model,” Repository Horizon University Indonesia, accessed October 31, 2025, https://repository.horizon.ac.id/items/show/1057.