Validasi Metode Identifikasi Fenilbutazon dan Deksametason dalam Jamu Pegal Linu secara Kromatografi Lapis Tipis-Densitometri
Dublin Core
Title
Validasi Metode Identifikasi Fenilbutazon dan Deksametason dalam Jamu Pegal Linu secara Kromatografi Lapis Tipis-Densitometri
Subject
Adulteration, Herbal Medicine, TLC
Description
Traditional herbal medicine, known as 'Jamu,' is prohibited from containing pharmaceutical chemical
substances (BKO) by the Indonesian Food and Drug Monitoring Agency (BPOM). In October 2022, BPOM
released a public warning after discovering the presence of phenylbutazone and dexamethasone in a muscle pain
relief jamu product. The purpose of this research is to establish a valid method for identifying phenylbutazone
and dexamethasone in jamu pegal linu. In this study, the method was applied to 10 brands of muscle pain relief
jamu samples. The samples were extracted with methanol and then spotted in a quantity of 5 μl on a silica gel 60
F254 plate, eluted with a selected mobile phase, namely ethyl acetate: methanol: ammonia (8.5:1:0.5, v/v/v).
Spots were observed with a densitometry at λ240 nm. Phenylbutazone appeared at Rf 0.33, and dexamethasone
at Rf 0.77, with a resolution value (Rs) of 5,8. The detection limits for phenylbutazone were 8,5307 μg/ml (0,1706
mg/600 mg capsule) and for dexamethasone, 8,7611 μg/ml (0,1752/600 mg capsule). The results revealed that
two samples tested positive for both phenylbutazone and dexamethasone, while six samples tested positive for dexamethasone.
substances (BKO) by the Indonesian Food and Drug Monitoring Agency (BPOM). In October 2022, BPOM
released a public warning after discovering the presence of phenylbutazone and dexamethasone in a muscle pain
relief jamu product. The purpose of this research is to establish a valid method for identifying phenylbutazone
and dexamethasone in jamu pegal linu. In this study, the method was applied to 10 brands of muscle pain relief
jamu samples. The samples were extracted with methanol and then spotted in a quantity of 5 μl on a silica gel 60
F254 plate, eluted with a selected mobile phase, namely ethyl acetate: methanol: ammonia (8.5:1:0.5, v/v/v).
Spots were observed with a densitometry at λ240 nm. Phenylbutazone appeared at Rf 0.33, and dexamethasone
at Rf 0.77, with a resolution value (Rs) of 5,8. The detection limits for phenylbutazone were 8,5307 μg/ml (0,1706
mg/600 mg capsule) and for dexamethasone, 8,7611 μg/ml (0,1752/600 mg capsule). The results revealed that
two samples tested positive for both phenylbutazone and dexamethasone, while six samples tested positive for dexamethasone.
Creator
Imelda Rindiantyas, Diana, Henry Kurnia Setiawan
Source
https://prosiding.arikesi.or.id/
Publisher
Asosiasi Riset Ilmu Kesehatan Indonesia
Date
Juni 2024
Contributor
Sri Wahyuni
Format
PDF
Language
Indonesian
Type
Text
Files
Citation
Imelda Rindiantyas, Diana, Henry Kurnia Setiawan, “Validasi Metode Identifikasi Fenilbutazon dan Deksametason dalam Jamu Pegal Linu secara Kromatografi Lapis Tipis-Densitometri,” Repository Horizon University Indonesia, accessed April 11, 2026, https://repository.horizon.ac.id/items/show/12089.