PROSIDING INTERNASIONAL KEPERAWATAN Proceedings of the International Conference on Nursing and Health Sciences, Volume 4 No 1
EFFECT OF PUMPKIN SOYGURT ON MICROBIOTA BALANCE IN DIABETES MELLITUS MODEL RATS
Dublin Core
Title
PROSIDING INTERNASIONAL KEPERAWATAN Proceedings of the International Conference on Nursing and Health Sciences, Volume 4 No 1
EFFECT OF PUMPKIN SOYGURT ON MICROBIOTA BALANCE IN DIABETES MELLITUS MODEL RATS
EFFECT OF PUMPKIN SOYGURT ON MICROBIOTA BALANCE IN DIABETES MELLITUS MODEL RATS
Subject
diabetes mellitus; escherichia coli; lactic acid bacteria; synbiotic
Description
Microbiota imbalance in the gastrointestinal tract causes low-grade systemic inflammation, which triggers
metabolic changes and insulin resistance to become diabetes mellitus. This condition can be controlled
through synbiotic supplementation through pumpkin soygurt containing lactic acid bacteria and
polysaccharides. This study aimed to analyze the effect of complementary therapy using pumpkin soygurt
and metformin on the abundance of lactic acid bacteria and Escherichia coli in a rat model of diabetes
mellitus. The research design is experimental with a post-test only. Male Wistar rats were divided into six
groups; healthy rats (KS), DM rats (K-), DM+metformin rats (K+), DM+metformin+1 mL pumpkin soygurt
rats (P1), DM+metformin+ 1.5 mL pumpkin soygurt rats (P2), DM + metformin + 2 mL of pumpkin soygurt
(P3) rats. The test material was given for four weeks. The abundance of LAB and Escherichia coli was
analyzed using a sample of the contents of the cecum with the method total plate count. Data were analyzed
using the One way ANOVA test. This study showed a dysbiosis condition on K- (BAL 6.85 CFU/mL;
Escherichia coli 8.80 CFU/mL) dan K+ (BAL 4,99 CFU/ml; Escherichia coli 7.67 CFU/mL). In the
treatment group, eubiotics were maintained, with the best conditions in group P2 with a BAL of 7.46
CFU/mL and Escherichia coli 4,62 CFU/mL. The combination of pumpkin soygurt and metformin can
maintain the balance of the gastrointestinal microbiota in DM model rats, especially lactic acid bacteria and
Escherichia coli.
metabolic changes and insulin resistance to become diabetes mellitus. This condition can be controlled
through synbiotic supplementation through pumpkin soygurt containing lactic acid bacteria and
polysaccharides. This study aimed to analyze the effect of complementary therapy using pumpkin soygurt
and metformin on the abundance of lactic acid bacteria and Escherichia coli in a rat model of diabetes
mellitus. The research design is experimental with a post-test only. Male Wistar rats were divided into six
groups; healthy rats (KS), DM rats (K-), DM+metformin rats (K+), DM+metformin+1 mL pumpkin soygurt
rats (P1), DM+metformin+ 1.5 mL pumpkin soygurt rats (P2), DM + metformin + 2 mL of pumpkin soygurt
(P3) rats. The test material was given for four weeks. The abundance of LAB and Escherichia coli was
analyzed using a sample of the contents of the cecum with the method total plate count. Data were analyzed
using the One way ANOVA test. This study showed a dysbiosis condition on K- (BAL 6.85 CFU/mL;
Escherichia coli 8.80 CFU/mL) dan K+ (BAL 4,99 CFU/ml; Escherichia coli 7.67 CFU/mL). In the
treatment group, eubiotics were maintained, with the best conditions in group P2 with a BAL of 7.46
CFU/mL and Escherichia coli 4,62 CFU/mL. The combination of pumpkin soygurt and metformin can
maintain the balance of the gastrointestinal microbiota in DM model rats, especially lactic acid bacteria and
Escherichia coli.
Creator
Annisa Avelia, Didik Gunawan Tamtomo, Yulia Sari
Source
http://jurnal.globalhealthsciencegroup.com/index.php/PICNHS
Publisher
Global Health Science Group
Date
January - June 2023
Contributor
PERI IRAWAN
Format
PDF
Language
ENGLISH
Type
TEXT
Files
Citation
Annisa Avelia, Didik Gunawan Tamtomo, Yulia Sari, “PROSIDING INTERNASIONAL KEPERAWATAN Proceedings of the International Conference on Nursing and Health Sciences, Volume 4 No 1
EFFECT OF PUMPKIN SOYGURT ON MICROBIOTA BALANCE IN DIABETES MELLITUS MODEL RATS,” Repository Horizon University Indonesia, accessed February 3, 2025, https://repository.horizon.ac.id/items/show/2801.
EFFECT OF PUMPKIN SOYGURT ON MICROBIOTA BALANCE IN DIABETES MELLITUS MODEL RATS,” Repository Horizon University Indonesia, accessed February 3, 2025, https://repository.horizon.ac.id/items/show/2801.