PROSIDING INTERNASIONAL KEPERAWATAN Proceedings of the International Conference on Nursing and Health Sciences Volume 1 No 1, November 2020
EVALUATION OF CYTOTOXIC ACTIVITY OF COMBINATION HONEY AND ALOE VERA IN NIH3T3 FIBROBLAST CELL LINES AND ITS EFFECT ON CELL VIABILITY
Dublin Core
Title
PROSIDING INTERNASIONAL KEPERAWATAN Proceedings of the International Conference on Nursing and Health Sciences Volume 1 No 1, November 2020
EVALUATION OF CYTOTOXIC ACTIVITY OF COMBINATION HONEY AND ALOE VERA IN NIH3T3 FIBROBLAST CELL LINES AND ITS EFFECT ON CELL VIABILITY
EVALUATION OF CYTOTOXIC ACTIVITY OF COMBINATION HONEY AND ALOE VERA IN NIH3T3 FIBROBLAST CELL LINES AND ITS EFFECT ON CELL VIABILITY
Subject
cytotoxic; combination honey and aloe vera; cell viability; in vitro
Description
Alternative treatments can be used singly or in mixture to treat and prevent disease. Honey and Aloe
vera are natural ingredients that have anti-microbial, anti-inflammatory, antioxidant properties that are
used to treat wounds. Animal studies have shown that giving honey and Aloe vera can speed up
wound healing time. This study aims to examine the cytotoxic effect of the combination of honey and
Aloe vera on the NIH3T3 cell line and its impact on cell viability. This research is a pure experiment
using the post-test only design with control group design. The cytotoxicity test was on NIH3T3
fibroblast cells using the MTT Assay method to determine the inhibitory concentration (IC50) value.
Cell viability was calculated using the MTT Assay method. The combination is divided into 5
concentrations, combination 1 (2% honey and 500 μg/mL (0.005%) Aloe vera)), combination 2 (1.5%
honey and 250μg/mL (0.0025%) Aloe vera)), combination 3 (1% honey and 125 μg/mL (0.00125%)
Aloe vera)), combination 4 (Honey 0.5% and 50μg/mL (0.0005%) Aloe vera)), combination 5 (Honey
0.1 % and 0.5μg/mL (0.00005%) Aloe vera)). The combination of honey and Aloe vera showed
inhibition below 13.87% with an IC50 value of 6.58%. Combination honey and Aloe vera on average
was able to increase the viability of fibroblast cells above 86.13%. Combination 4 and 5 can increase
cell viability 96.50% and 99.80%. The combination of honey and Aloe vera does not cause cytotoxic
effects on NIH3T3 fibroblasts cell line and can increase the viability of fibroblasts.
vera are natural ingredients that have anti-microbial, anti-inflammatory, antioxidant properties that are
used to treat wounds. Animal studies have shown that giving honey and Aloe vera can speed up
wound healing time. This study aims to examine the cytotoxic effect of the combination of honey and
Aloe vera on the NIH3T3 cell line and its impact on cell viability. This research is a pure experiment
using the post-test only design with control group design. The cytotoxicity test was on NIH3T3
fibroblast cells using the MTT Assay method to determine the inhibitory concentration (IC50) value.
Cell viability was calculated using the MTT Assay method. The combination is divided into 5
concentrations, combination 1 (2% honey and 500 μg/mL (0.005%) Aloe vera)), combination 2 (1.5%
honey and 250μg/mL (0.0025%) Aloe vera)), combination 3 (1% honey and 125 μg/mL (0.00125%)
Aloe vera)), combination 4 (Honey 0.5% and 50μg/mL (0.0005%) Aloe vera)), combination 5 (Honey
0.1 % and 0.5μg/mL (0.00005%) Aloe vera)). The combination of honey and Aloe vera showed
inhibition below 13.87% with an IC50 value of 6.58%. Combination honey and Aloe vera on average
was able to increase the viability of fibroblast cells above 86.13%. Combination 4 and 5 can increase
cell viability 96.50% and 99.80%. The combination of honey and Aloe vera does not cause cytotoxic
effects on NIH3T3 fibroblasts cell line and can increase the viability of fibroblasts.
Creator
Januar Rizqi, Tia Amestiasih
Source
http://jurnal.globalhealthsciencegroup.com/index.php/PICNHS
Publisher
Global Health Science Group
Date
November 2020
Contributor
PERI IRAWAN
Format
PDF
Language
ENGLISH
Type
TEXT
Files
Citation
Januar Rizqi, Tia Amestiasih, “PROSIDING INTERNASIONAL KEPERAWATAN Proceedings of the International Conference on Nursing and Health Sciences Volume 1 No 1, November 2020
EVALUATION OF CYTOTOXIC ACTIVITY OF COMBINATION HONEY AND ALOE VERA IN NIH3T3 FIBROBLAST CELL LINES AND ITS EFFECT ON CELL VIABILITY,” Repository Horizon University Indonesia, accessed April 3, 2025, https://repository.horizon.ac.id/items/show/2990.
EVALUATION OF CYTOTOXIC ACTIVITY OF COMBINATION HONEY AND ALOE VERA IN NIH3T3 FIBROBLAST CELL LINES AND ITS EFFECT ON CELL VIABILITY,” Repository Horizon University Indonesia, accessed April 3, 2025, https://repository.horizon.ac.id/items/show/2990.