PROCEEDINGS 3nd INTERNATIONAL CONFERENCE ON HANDLING NON-COMMUNICABLE DISEASES (3ndICHNCDs) -A Web International Conference- 2021
Politeknik Kesehatan Kemenkes Semarang : “The Role of Health Care Providers on Handling Non-Communicable Disease Through Innovative Technology in the Research.”
CYTOTOXIC EFFECTS OF POGONATUM CIRRATUM EXTRACTS ON CCL-119 HUMAN ACUTE LYMPHOBLASTIC LEUKAEMIC CELL LINE
Dublin Core
Title
PROCEEDINGS 3nd INTERNATIONAL CONFERENCE ON HANDLING NON-COMMUNICABLE DISEASES (3ndICHNCDs) -A Web International Conference- 2021
Politeknik Kesehatan Kemenkes Semarang : “The Role of Health Care Providers on Handling Non-Communicable Disease Through Innovative Technology in the Research.”
CYTOTOXIC EFFECTS OF POGONATUM CIRRATUM EXTRACTS ON CCL-119 HUMAN ACUTE LYMPHOBLASTIC LEUKAEMIC CELL LINE
Politeknik Kesehatan Kemenkes Semarang : “The Role of Health Care Providers on Handling Non-Communicable Disease Through Innovative Technology in the Research.”
CYTOTOXIC EFFECTS OF POGONATUM CIRRATUM EXTRACTS ON CCL-119 HUMAN ACUTE LYMPHOBLASTIC LEUKAEMIC CELL LINE
Subject
cytotoxicity, lymphocyte, chemotherapy, phytochemical
Description
Due to various harmful adverse effects associated with the current treatment regimen of childhood acute lymphoblastic leukaemia (ALL), natural products have become the focus of many studies looking for alternative milder anticancer agents. This study aimed to investigate the phytochemicals and cytotoxic activity of bryophyte moss Pogonatum cirratum subsp. macrophyllum extracts on CCL-119 human T-cell ALL (T-ALL) cell line. P. cirratum was extracted using three solvents of different polarities, i.e., aqueous, methanol, and petroleum ether through the
sonication technique. Each extract was then chemically subjected to a phytochemical screening of phenol, flavonoid, alkaloid, tannin, coumarin, saponin, terpenoid, and glycoside. CCL-119 T-ALL and normal B lymphocyte (WIL2-NS) cell lines were treated with aqueous, methanol, and petroleum ether extracts at different doses ranging from 0–200 µg/mL for 48 hours. Cytotoxicity effect of the extracts against both cell lines was assessed using WST-1 cell proliferation assay. Dexamethasone, a clinical ALL chemotherapy, was used as a positive control.
The percentage yield of aqueous, methanol, and petroleum ether extracts were 1.4%, 6.25%, and 0.38%, respectively. Collectively, the extracts were tested positive for flavonoid, glycoside, phenol, and saponin. P.cirratum exerted its cytotoxic effect on CCL-119 cells, with aqueous extract being the most potent, followed by methanol and petroleum ether extracts, due to the antiproliferative phytochemical contents. No toxic effect was
observed in normal WIL2-NS cells. Conclusively, the cytotoxic effect of P. cirratum in cultured leukaemic cell provides a scientific basis for its usefulness as a potential ALL chemotherapeutic agent.
sonication technique. Each extract was then chemically subjected to a phytochemical screening of phenol, flavonoid, alkaloid, tannin, coumarin, saponin, terpenoid, and glycoside. CCL-119 T-ALL and normal B lymphocyte (WIL2-NS) cell lines were treated with aqueous, methanol, and petroleum ether extracts at different doses ranging from 0–200 µg/mL for 48 hours. Cytotoxicity effect of the extracts against both cell lines was assessed using WST-1 cell proliferation assay. Dexamethasone, a clinical ALL chemotherapy, was used as a positive control.
The percentage yield of aqueous, methanol, and petroleum ether extracts were 1.4%, 6.25%, and 0.38%, respectively. Collectively, the extracts were tested positive for flavonoid, glycoside, phenol, and saponin. P.cirratum exerted its cytotoxic effect on CCL-119 cells, with aqueous extract being the most potent, followed by methanol and petroleum ether extracts, due to the antiproliferative phytochemical contents. No toxic effect was
observed in normal WIL2-NS cells. Conclusively, the cytotoxic effect of P. cirratum in cultured leukaemic cell provides a scientific basis for its usefulness as a potential ALL chemotherapeutic agent.
Creator
Elda Surhaida Latif, Rajayaashini Rajasegaran, Nik Norhazrina Nik Mohd Kamil, Nur Syazwana Muzani, Asyifaa Syahirah Md Padzil, Nurul Farahana Kamaludin , and Normah Awang
Publisher
Politeknik Kesehatan Kemenkes Semarang
Date
23rd - 24th November 2021
Contributor
Sri Wahyuni
Rights
ISBN: 978-623-5709-13-0
Format
PDF
Language
English
Type
Text
Coverage
Prosiding Politeknik Kesehatan Kemenkes Semarang 2021
Files
Citation
Elda Surhaida Latif, Rajayaashini Rajasegaran, Nik Norhazrina Nik Mohd Kamil, Nur Syazwana Muzani, Asyifaa Syahirah Md Padzil, Nurul Farahana Kamaludin , and Normah Awang, “PROCEEDINGS 3nd INTERNATIONAL CONFERENCE ON HANDLING NON-COMMUNICABLE DISEASES (3ndICHNCDs) -A Web International Conference- 2021
Politeknik Kesehatan Kemenkes Semarang : “The Role of Health Care Providers on Handling Non-Communicable Disease Through Innovative Technology in the Research.”
CYTOTOXIC EFFECTS OF POGONATUM CIRRATUM EXTRACTS ON CCL-119 HUMAN ACUTE LYMPHOBLASTIC LEUKAEMIC CELL LINE,” Repository Horizon University Indonesia, accessed September 20, 2024, https://repository.horizon.ac.id/items/show/370.
Politeknik Kesehatan Kemenkes Semarang : “The Role of Health Care Providers on Handling Non-Communicable Disease Through Innovative Technology in the Research.”
CYTOTOXIC EFFECTS OF POGONATUM CIRRATUM EXTRACTS ON CCL-119 HUMAN ACUTE LYMPHOBLASTIC LEUKAEMIC CELL LINE,” Repository Horizon University Indonesia, accessed September 20, 2024, https://repository.horizon.ac.id/items/show/370.