Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020
Zika, Chikungunya, and Dengue Viral Infections in Human Peripheral Blood Mononuclear Cells: Cell Susceptibility and Gene Expression
Dublin Core
Title
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020
Zika, Chikungunya, and Dengue Viral Infections in Human Peripheral Blood Mononuclear Cells: Cell Susceptibility and Gene Expression
Zika, Chikungunya, and Dengue Viral Infections in Human Peripheral Blood Mononuclear Cells: Cell Susceptibility and Gene Expression
Subject
chemokines, chikungunya virus, cytokines, dengue virus, mononuclear leukocytes, Zika virus
Description
BACKGROUND Infections of Zika (ZIKV), dengue (DENV), and chikungunya viruses (CHIKV) are presented with similar clinical symptoms; these often lead to misdiagnosis. Viremia levels and host immune responses may contribute to disease severity. This study was aimed to characterize the ability of ZIKV, CHIKV, and DENV to infect human
peripheral blood mononuclear cells (PBMCs) and assess the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-10, and interferon gamma-induced protein (IP)-10 genes in response to the viral infections.
METHODS PBMCs were isolated from healthy donors using gradient centrifugation. Cells were infected with Indonesian isolates of ZIKV, CHIKV, and DENV for 48 hours. Plaque assays were performed to measure viable virus titers, while viral genomic RNA and the gene expression of TNF-α, IL-10, and IP-10 were determined using real-time
quantitative reverse transcription-polymerase chain reaction.
RESULTS The susceptibility of PBMCs to ZIKV, CHIKV, and DENV infection was observed, and the viable virus titer and viral genome quantity were found to be significantly higher in ZIKV and CHIKV. All viruses induced the expression of immune-related proteins. The TNF-α gene was upregulated by all viruses to relatively similar
levels. IL-10 expression was highest in response to ZIKV, followed by CHIKV. In contrast,
IP-10 expression was highly upregulated in DENV-infected cells and only moderately
expressed in ZIKV- and CHIKV-infected cells.
CONCLUSIONS ZIKV, CHIKV, and DENV clinical isolates infected PBMCs with different
levels of virus infectivity. The gene expression of IL-10 was highly upregulated in ZIKV
infection and IP-10 in DENV infection.
peripheral blood mononuclear cells (PBMCs) and assess the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-10, and interferon gamma-induced protein (IP)-10 genes in response to the viral infections.
METHODS PBMCs were isolated from healthy donors using gradient centrifugation. Cells were infected with Indonesian isolates of ZIKV, CHIKV, and DENV for 48 hours. Plaque assays were performed to measure viable virus titers, while viral genomic RNA and the gene expression of TNF-α, IL-10, and IP-10 were determined using real-time
quantitative reverse transcription-polymerase chain reaction.
RESULTS The susceptibility of PBMCs to ZIKV, CHIKV, and DENV infection was observed, and the viable virus titer and viral genome quantity were found to be significantly higher in ZIKV and CHIKV. All viruses induced the expression of immune-related proteins. The TNF-α gene was upregulated by all viruses to relatively similar
levels. IL-10 expression was highest in response to ZIKV, followed by CHIKV. In contrast,
IP-10 expression was highly upregulated in DENV-infected cells and only moderately
expressed in ZIKV- and CHIKV-infected cells.
CONCLUSIONS ZIKV, CHIKV, and DENV clinical isolates infected PBMCs with different
levels of virus infectivity. The gene expression of IL-10 was highly upregulated in ZIKV
infection and IP-10 in DENV infection.
Creator
Ahmad Husein Alkaff, Benediktus Yohan, Usman Sumo Friend Tambunan, R. Tedjo Sasmono
Publisher
Fakultas Kedokteran Universitas Indonesia
Date
2020-07-02
Contributor
Sri Wahyuni
Rights
ISSN : 0853-1773
Format
PDF
Language
English
Type
Text
Coverage
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020
Files
Citation
Ahmad Husein Alkaff, Benediktus Yohan, Usman Sumo Friend Tambunan, R. Tedjo Sasmono, “Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020
Zika, Chikungunya, and Dengue Viral Infections in Human Peripheral Blood Mononuclear Cells: Cell Susceptibility and Gene Expression,” Repository Horizon University Indonesia, accessed October 31, 2025, https://repository.horizon.ac.id/items/show/863.
Zika, Chikungunya, and Dengue Viral Infections in Human Peripheral Blood Mononuclear Cells: Cell Susceptibility and Gene Expression,” Repository Horizon University Indonesia, accessed October 31, 2025, https://repository.horizon.ac.id/items/show/863.