Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020
MLH1 and MSH2 Mismatch Repair Protein Profile using Immunohistochemistry in Nepalese Colorectal Cancer Patients
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Title
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020
MLH1 and MSH2 Mismatch Repair Protein Profile using Immunohistochemistry in Nepalese Colorectal Cancer Patients
MLH1 and MSH2 Mismatch Repair Protein Profile using Immunohistochemistry in Nepalese Colorectal Cancer Patients
Subject
colorectal cancer, immunohistochemistry, mismatch repair, MutL homolog 1, MutS homolog 2
Description
BACKGROUND Hereditary nonpolyposis colorectal cancer, or Lynch syndrome, caused by germline mutations or genetic defects in mismatch repair (MMR) genes (MLH1, MSH2, PMS2, MSH6, and epithelial cellular adhesion molecule), is an autosomal dominant condition accounting for 2–5% of all colorectal carcinomas (CRCs). Reports on MMR loss in many populations are available; however, there are no reports on the frequency
of MMR protein expression in Nepalese cohorts. Therefore, this study was aimed to assess the expression profiles of MLH1 and MSH2 protein by immunohistochemistry (IHC) in Nepalese CRC patients.
METHODS This retrospective study used archived formalin-fixed paraffin-embedded tissue blocks from 43 Nepalese CRC patients. IHC staining was performed using MLH1 and MSH2 antibodies. IHC scoring analysis was assessed using semiquantitative scoring.
RESULTS Of the 43 CRC patients, 8 (18.6%) showed loss of staining for MLH1 antibody, 5 (11.6%) showed loss of staining for MSH2 antibody, and 4 (9.3%) showed loss of staining for both MLH1 and MSH2 antibodies.
CONCLUSIONS IHC is a potential screening method of determining the MMR expression
profile of Nepalese CRC patients. IHC can be performed in local clinical laboratories to find MMR protein defects in selected cases prior to expensive molecular tests.
of MMR protein expression in Nepalese cohorts. Therefore, this study was aimed to assess the expression profiles of MLH1 and MSH2 protein by immunohistochemistry (IHC) in Nepalese CRC patients.
METHODS This retrospective study used archived formalin-fixed paraffin-embedded tissue blocks from 43 Nepalese CRC patients. IHC staining was performed using MLH1 and MSH2 antibodies. IHC scoring analysis was assessed using semiquantitative scoring.
RESULTS Of the 43 CRC patients, 8 (18.6%) showed loss of staining for MLH1 antibody, 5 (11.6%) showed loss of staining for MSH2 antibody, and 4 (9.3%) showed loss of staining for both MLH1 and MSH2 antibodies.
CONCLUSIONS IHC is a potential screening method of determining the MMR expression
profile of Nepalese CRC patients. IHC can be performed in local clinical laboratories to find MMR protein defects in selected cases prior to expensive molecular tests.
Creator
Matrika Bhattarai, Wan Khairunnisa Wan Juhari, Raju Lama, Chin Bahadur Pun, Wardah Yusof, Wan Faiziah Wan Abdul Rahman, Andee Dzulkarnaen Zakaria, Khairul Bariah Ahmad Amin Noordin, Tilak R. Shrestha, Bin Alwi Zilfalil
Publisher
Fakultas Kedokteran Universitas Indonesia
Date
2020-07-02
Contributor
Sri Wahyuni
Rights
ISSN : 0853-1773
Format
PDF
Language
English
Type
Text
Coverage
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020
Files
Citation
Matrika Bhattarai, Wan Khairunnisa Wan Juhari, Raju Lama, Chin Bahadur Pun, Wardah Yusof, Wan Faiziah Wan Abdul Rahman, Andee Dzulkarnaen Zakaria, Khairul Bariah Ahmad Amin Noordin, Tilak R. Shrestha, Bin Alwi Zilfalil, “Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 2 2020
MLH1 and MSH2 Mismatch Repair Protein Profile using Immunohistochemistry in Nepalese Colorectal Cancer Patients,” Repository Horizon University Indonesia, accessed November 14, 2024, https://repository.horizon.ac.id/items/show/864.
MLH1 and MSH2 Mismatch Repair Protein Profile using Immunohistochemistry in Nepalese Colorectal Cancer Patients,” Repository Horizon University Indonesia, accessed November 14, 2024, https://repository.horizon.ac.id/items/show/864.