Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 4 2020
Tear Hypoxia-Inducible Factor-1α Expression, Lactate Dehydrogenase, and Malate Dehydrogenase Activity Changes in Soft Contact Lens Wear

Dublin Core

Title

Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 4 2020
Tear Hypoxia-Inducible Factor-1α Expression, Lactate Dehydrogenase, and Malate Dehydrogenase Activity Changes in Soft Contact Lens Wear

Subject

contact lenses, cornea, hypoxia, hypoxia-inducible factor 1, lactate dehydrogenase, malate dehydrogenase

Description

BACKGROUND Soft contact lens (SCL) wear can lead to a corneal hypoxia. However, there is a lack of studies looking for corneal hypoxia biomarkers in tear. This study aimed to investigate corneal hypoxia among SCL wearers based on hypoxia-inducible factor1α (HIF-1α) expression, tear lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) activities.
METHODS A nonrandomized clinical trial was conducted on two groups. SCLs were prescribed for 2 months to a group of new wearers. Meanwhile, SCL wear was discontinued for 1 month in a group of long-term wearers. Tear samples were then
collected on days 1, 7, 14, 28, and 56 after treatment. Repeated-measures analysis of
variance and Friedman’s test with post-hoc statistical analysis were used to evaluate
biomolecular changes (HIF-1α concentration, LDH, and MDH activities) in both groups.
RESULTS A total of 14 subjects (28 eyes) were enrolled in each group. In new wearers, there was a significant decrease in MDH level (p = 0.010) and no effect on HIF-1α level. In long-term wearers, HIF-1α and LDH levels tended to decrease (p = 0.054). A
significant decrease on MDH level was noted on days 7 (p = 0.003), 14 (p = 0.026), and 28 (p<0.010). Long-term wearers had a higher LDH baseline level than new wearers (p= 0.04).
CONCLUSIONS Corneal hypoxia was not proven after 2 months of SCL wear using biomarkers. However, LDH and MDH activities in tears were found to be decline after SCL discontinuation.

Creator

Tri Rahayu, Tjahjono Darminto Gondhowiardjo, Widya Artini, Septelia Inawati Wanandi

Publisher

Fakultas Kedokteran Universitas Indonesia

Date

2021-01-01

Contributor

Sri Wahyuni

Rights

ISSN : 0853-1773

Format

PDF

Language

English

Type

Text

Coverage

Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 4 2020

Files

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,Repository, Repository Horizon University Indonesia, Repository Universitas Horizon Indonesia, Horizon.ac.id, Horizon University Indonesia, Universitas Horizon Indonesia, HorizonU, Repo Horizon , ,Repository, Repository Horizon University Indonesia, Repository Universitas Horizon Indonesia, Horizon.ac.id, Horizon University Indonesia, Universitas Horizon Indonesia, HorizonU, Repo Horizon , ,Repository, Repository Horizon University Indonesia, Repository Universitas Horizon Indonesia, Horizon.ac.id, Horizon University Indonesia, Universitas Horizon Indonesia, HorizonU, Repo Horizon , ,Repository, Repository Horizon University Indonesia, Repository Universitas Horizon Indonesia, Horizon.ac.id, Horizon University Indonesia, Universitas Horizon Indonesia, HorizonU, Repo Horizon , ,Repository, Repository Horizon University Indonesia, Repository Universitas Horizon Indonesia, Horizon.ac.id, Horizon University Indonesia, Universitas Horizon Indonesia, HorizonU, Repo Horizon , ,Repository, Repository Horizon University Indonesia, Repository Universitas Horizon Indonesia, Horizon.ac.id, Horizon University Indonesia, Universitas Horizon Indonesia, HorizonU, Repo Horizon ,

Citation

Tri Rahayu, Tjahjono Darminto Gondhowiardjo, Widya Artini, Septelia Inawati Wanandi, “Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 4 2020
Tear Hypoxia-Inducible Factor-1α Expression, Lactate Dehydrogenase, and Malate Dehydrogenase Activity Changes in Soft Contact Lens Wear,” Repository Horizon University Indonesia, accessed November 21, 2024, https://repository.horizon.ac.id/items/show/901.