Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 4 2020
Tear Hypoxia-Inducible Factor-1α Expression, Lactate Dehydrogenase, and Malate Dehydrogenase Activity Changes in Soft Contact Lens Wear
Dublin Core
Title
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 4 2020
Tear Hypoxia-Inducible Factor-1α Expression, Lactate Dehydrogenase, and Malate Dehydrogenase Activity Changes in Soft Contact Lens Wear
Tear Hypoxia-Inducible Factor-1α Expression, Lactate Dehydrogenase, and Malate Dehydrogenase Activity Changes in Soft Contact Lens Wear
Subject
contact lenses, cornea, hypoxia, hypoxia-inducible factor 1, lactate dehydrogenase, malate dehydrogenase
Description
BACKGROUND Soft contact lens (SCL) wear can lead to a corneal hypoxia. However, there is a lack of studies looking for corneal hypoxia biomarkers in tear. This study aimed to investigate corneal hypoxia among SCL wearers based on hypoxia-inducible factor1α (HIF-1α) expression, tear lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) activities.
METHODS A nonrandomized clinical trial was conducted on two groups. SCLs were prescribed for 2 months to a group of new wearers. Meanwhile, SCL wear was discontinued for 1 month in a group of long-term wearers. Tear samples were then
collected on days 1, 7, 14, 28, and 56 after treatment. Repeated-measures analysis of
variance and Friedman’s test with post-hoc statistical analysis were used to evaluate
biomolecular changes (HIF-1α concentration, LDH, and MDH activities) in both groups.
RESULTS A total of 14 subjects (28 eyes) were enrolled in each group. In new wearers, there was a significant decrease in MDH level (p = 0.010) and no effect on HIF-1α level. In long-term wearers, HIF-1α and LDH levels tended to decrease (p = 0.054). A
significant decrease on MDH level was noted on days 7 (p = 0.003), 14 (p = 0.026), and 28 (p<0.010). Long-term wearers had a higher LDH baseline level than new wearers (p= 0.04).
CONCLUSIONS Corneal hypoxia was not proven after 2 months of SCL wear using biomarkers. However, LDH and MDH activities in tears were found to be decline after SCL discontinuation.
METHODS A nonrandomized clinical trial was conducted on two groups. SCLs were prescribed for 2 months to a group of new wearers. Meanwhile, SCL wear was discontinued for 1 month in a group of long-term wearers. Tear samples were then
collected on days 1, 7, 14, 28, and 56 after treatment. Repeated-measures analysis of
variance and Friedman’s test with post-hoc statistical analysis were used to evaluate
biomolecular changes (HIF-1α concentration, LDH, and MDH activities) in both groups.
RESULTS A total of 14 subjects (28 eyes) were enrolled in each group. In new wearers, there was a significant decrease in MDH level (p = 0.010) and no effect on HIF-1α level. In long-term wearers, HIF-1α and LDH levels tended to decrease (p = 0.054). A
significant decrease on MDH level was noted on days 7 (p = 0.003), 14 (p = 0.026), and 28 (p<0.010). Long-term wearers had a higher LDH baseline level than new wearers (p= 0.04).
CONCLUSIONS Corneal hypoxia was not proven after 2 months of SCL wear using biomarkers. However, LDH and MDH activities in tears were found to be decline after SCL discontinuation.
Creator
Tri Rahayu, Tjahjono Darminto Gondhowiardjo, Widya Artini, Septelia Inawati Wanandi
Publisher
Fakultas Kedokteran Universitas Indonesia
Date
2021-01-01
Contributor
Sri Wahyuni
Rights
ISSN : 0853-1773
Format
PDF
Language
English
Type
Text
Coverage
Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 4 2020
Files
Citation
Tri Rahayu, Tjahjono Darminto Gondhowiardjo, Widya Artini, Septelia Inawati Wanandi, “Jurnal Internasional Medical Journal of Indonesia FKUI Vol. 29 No. 4 2020
Tear Hypoxia-Inducible Factor-1α Expression, Lactate Dehydrogenase, and Malate Dehydrogenase Activity Changes in Soft Contact Lens Wear,” Repository Horizon University Indonesia, accessed November 10, 2024, https://repository.horizon.ac.id/items/show/901.
Tear Hypoxia-Inducible Factor-1α Expression, Lactate Dehydrogenase, and Malate Dehydrogenase Activity Changes in Soft Contact Lens Wear,” Repository Horizon University Indonesia, accessed November 10, 2024, https://repository.horizon.ac.id/items/show/901.